Internal amino acid sequencing service
The internal amino acid sequencing service is applied to protein samples delivered lyophilzed, in solution or bound to PVDF membrane. Depending on the requested type of service we digest the protein using different endoproteases and/or chemical protein cleavage. The generated peptides are separated and fractionated using an Agilent 1100 capillary HPLC system with microfraction collector ensuring highest sensitivity. Peptide peaks are applied to N-terminal sequencing with or without additional mass spectrometrical analyses like determination of peptide mass, MSMS or de novo peptide sequencing.
Services:
N-terminal and internal Edman sequencing service
Complete sequencing of proteins
N-terminal and internal Edman sequencing service:
N-terminal protein & peptide sequencing
Common:
Procise - N-terminal Edman Protein Sequencing
Edman PTH Amino Acid Standard
Step 1 - Proline
Step 2 - Phenylalanine
Step 3 - Arginine
Step 4 - Serine
Step 5 - Alanine
Step 6 - Leucine
